Hypusination Maintains Intestinal Homeostasis and Prevents Colitis and Carcinogenesis by Enhancing Aldehyde Detoxification.

ABSTRACT:

Background & aims

The amino acid hypusine, synthesized from the polyamine spermidine by the enzyme deoxyhypusine synthase (DHPS), is essential for the activity of eukaryotic translation initiation factor 5A (EIF5A). The role of hypusinated EIF5A (EIF5A^Hyp) remains unknown in intestinal homeostasis. Our aim was to investigate EIF5A^Hyp in the gut epithelium in inflammation and carcinogenesis.

Methods

We used human colon tissue messenger RNA samples and publicly available transcriptomic datasets, tissue microarrays, and patient-derived colon organoids. Mice with intestinal epithelial-specific deletion of Dhps were investigated at baseline and in models of colitis and colon carcinogenesis.

Results

We found that patients with ulcerative colitis and Cr

SUBMITTER: Gobert AP 

PROVIDER: S-EPMC10527201 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature