Dataset Information

Functional Interaction between Adenosine A_2A and mGlu₅ Receptors Mediates STEP Phosphatase Activation and Promotes STEP/mGlu₅R Binding in Mouse Hippocampus and Neuroblastoma Cell Line.

ABSTRACT: (1) Background: Recently, we found that adenosine A_2A receptor (A_2AR) stimulation results in an increase in STEP phosphatase activity. In order to delve into the mechanism through which A_2AR stimulation induced STEP activation, we investigated the involvement of mGlu₅R since it is well documented that A_2AR and mGlu₅R physically and functionally interact in several brain areas. (2) Methods: In a neuroblastoma cell line (SH-SY5Y) and in mouse hippocampal slices, we evaluated the enzymatic activity of STEP by using a para-nitrophenyl phosphate colorimetric assay. A co-immunoprecipitation assay and a Western blot analysis were used to evaluate STEP/mGlu₅R binding. (3) Results: We found that the A_2AR-depende

SUBMITTER: Mallozzi C

PROVIDER: S-EPMC10527457 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature

ACCESS DATA

Dataset Information

Functional Interaction between Adenosine A_2A and mGlu₅ Receptors Mediates STEP Phosphatase Activation and Promotes STEP/mGlu₅R Binding in Mouse Hippocampus and Neuroblastoma Cell Line.

OmicsDI is part of the ELIXIR infrastructure

Tweets

Dataset Information

Functional Interaction between Adenosine A2A and mGlu5 Receptors Mediates STEP Phosphatase Activation and Promotes STEP/mGlu5R Binding in Mouse Hippocampus and Neuroblastoma Cell Line.

OmicsDI is part of the ELIXIR infrastructure

Tweets

Functional Interaction between Adenosine A_2A and mGlu₅ Receptors Mediates STEP Phosphatase Activation and Promotes STEP/mGlu₅R Binding in Mouse Hippocampus and Neuroblastoma Cell Line.