Cardiac Pericytes Acquire a Fibrogenic Phenotype and Contribute to Vascular Maturation After Myocardial Infarction.

ABSTRACT:

Background

Pericytes have been implicated in tissue repair, remodeling, and fibrosis. Although the mammalian heart contains abundant pericytes, their fate and involvement in myocardial disease remains unknown.

Methods

We used NG2^Dsred;PDGFRα^EGFP pericyte:fibroblast dual reporter mice and inducible NG2^CreER mice to study the fate and phenotypic modulation of pericytes in myocardial infarction. The transcriptomic profile of pericyte-derived cells was studied using polymerase chain reaction arrays and single-cell RNA sequencing. The role of transforming growth factor-β (TGF-β) signaling in regulation of pericyte phenotype was investigated in vivo using pericyte-specific TGF-β receptor 2 knockout mice and in vitro using cultured human placental p

SUBMITTER: Alex L 

PROVIDER: S-EPMC10527624 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature