OBOX regulates mouse zygotic genome activation and early development.

ABSTRACT: Zygotic genome activation (ZGA) activates the quiescent genome to enable the maternal-to-zygotic transition^1,2. However, the identity of transcription factors that underlie mammalian ZGA in vivo remains elusive. Here we show that OBOX, a PRD-like homeobox domain transcription factor family (OBOX1-OBOX8)^3-5, are key regulators of mouse ZGA. Mice deficient for maternally transcribed Obox1/2/5/7 and zygotically expressed Obox3/4 had a two-cell to four-cell arrest, accompanied by impaired ZGA. The Obox knockout defects could be rescued by restoring either maternal and zygotic OBOX, which suggests that maternal and zygotic OBOX redundantly support embryonic development. Chromatin-binding analysis showed that Obox knockout preferentially affected OBOX-binding targets. Mecha

SUBMITTER: Ji S 

PROVIDER: S-EPMC10528489 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature