Single-Molecular Dissection of Liquid-Liquid Phase Transitions.

ABSTRACT: Interaction between peptides and nucleic acids is a ubiquitous process that drives many cellular functions, such as replications, transcriptions, and translations. Recently, this interaction has been found in liquid-liquid phase separation (LLPS), a process responsible for the formation of newly discovered membraneless organelles with a variety of biological functions inside cells. In this work, we studied the molecular interaction between the poly-l-lysine (PLL) peptide and nucleic acids during the early stage of an LLPS process at the single-molecule level using optical tweezers. By monitoring the mechanical tension of individual nucleic acid templates upon PLL addition, we revealed a multistage LLPS process mediated by the long-range interactions between nucleic acids and polyelectrolyt

SUBMITTER: Pokhrel P 

PROVIDER: S-EPMC10528544 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature