Airway stem cell reconstitution by the transplantation of primary or pluripotent stem cell-derived basal cells.

ABSTRACT: Life-long reconstitution of a tissue's resident stem cell compartment with engrafted cells has the potential to durably replenish organ function. Here, we demonstrate the engraftment of the airway epithelial stem cell compartment via intra-airway transplantation of mouse or human primary and pluripotent stem cell (PSC)-derived airway basal cells (BCs). Murine primary or PSC-derived BCs transplanted into polidocanol-injured syngeneic recipients give rise for at least two years to progeny that stably display the morphologic, molecular, and functional phenotypes of airway epithelia. The engrafted basal-like cells retain extensive self-renewal potential, evident by the capacity to reconstitute the tracheal epithelium through seven generations of secondary transplantation. Using the same approa

SUBMITTER: Ma L 

PROVIDER: S-EPMC10528754 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature