Discoveries of the specific expression of lncRNAs and mRNAs in hippocampus of rats after traumatic brain injury.

ABSTRACT:

Objects

Explore the relationship between the neural function deficit and the changes of lncRNA and mRNA in hippocampus after traumatic brain injury (TBI) in rats.

Methods

Twenty male rats weighted 200-240 grams were randomly divided into sham group and TBI group. Neurologic severity score (NSS) was performed after operation, and the hippocampus of rats was collected for long non-coding RNAs (lncRNAs), mRNAs microarray detection, real-time quantitative PCR Detecting System (Q-PCR), western blot (WB) detection, and serum biochemical detection.

Results

The NSS score of the TBI group was significantly higher than the sham group. Compared with the sham group, 270 lncRNAs changed in the TBI group, of which 224 were up-regulated and 46 were down-regulated. Among up-regulated

SUBMITTER: Li J 

PROVIDER: S-EPMC10528755 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature