Spatial organization and function of RNA molecules within phase-separated condensates in zebrafish are controlled by Dnd1.

ABSTRACT: Germ granules, condensates of phase-separated RNA and protein, are organelles that are essential for germline development in different organisms. The patterning of the granules and their relevance for germ cell fate are not fully understood. Combining three-dimensional in vivo structural and functional analyses, we study the dynamic spatial organization of molecules within zebrafish germ granules. We find that the localization of RNA molecules to the periphery of the granules, where ribosomes are localized, depends on translational activity at this location. In addition, we find that the vertebrate-specific Dead end (Dnd1) protein is essential for nanos3 RNA localization at the condensates' periphery. Accordingly, in the absence of Dnd1, or when translation is inhibited, nanos3 RNA translo

SUBMITTER: Westerich KJ 

PROVIDER: S-EPMC10528888 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature