Miniaturized microarray-format digital ELISA enabled by lithographic protein patterning.

ABSTRACT: The search for reliable protein biomarker candidates is critical for early disease detection and treatment. However, current immunoassay technologies are failing to meet increasing demands for sensitivity and multiplexing. Here, the authors have created a highly sensitive protein microarray using the principle of single-molecule counting for signal amplification, capable of simultaneously detecting a panel of cancer biomarkers at sub-pg/mL levels. To enable this amplification strategy, the authors introduce a novel method of protein patterning using photolithography to subdivide addressable arrays of capture antibody spots into hundreds of thousands of individual microwells. This allows for the total sensor area to be miniaturized, increasing the total possible multiplex capacity. With the

SUBMITTER: Stephens AD 

PROVIDER: S-EPMC10528924 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature