Epigenetic Induction of Smooth Muscle Cell Phenotypic Alterations in Aortic Aneurysms and Dissections.

ABSTRACT:

Background

Smooth muscle cell (SMC) phenotypic switching has been increasingly detected in aortic aneurysm and dissection (AAD) tissues. However, the diverse SMC phenotypes in AAD tissues and the mechanisms driving SMC phenotypic alterations remain to be identified.

Methods

We examined the transcriptomic and epigenomic dynamics of aortic SMC phenotypic changes in mice with angiotensin II-induced AAD by using single-cell RNA sequencing and single-cell sequencing assay for transposase-accessible chromatin. SMC phenotypic alteration in aortas from patients with ascending thoracic AAD was examined by using single-cell RNA sequencing analysis.

Results

Single-cell RNA sequencing analysis revealed that aortic stress induced the transition of SMCs from a primary contractile p

SUBMITTER: Chakraborty A 

PROVIDER: S-EPMC10529114 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature