Tracking B cell responses to the SARS-CoV-2 mRNA-1273 vaccine.

ABSTRACT: Protective immunity following vaccination is sustained by long-lived antibody-secreting cells and resting memory B cells (MBCs). Responses to two-dose SARS-CoV-2 mRNA-1273 vaccination are evaluated longitudinally by multimodal single-cell analysis in three infection-naïve individuals. Integrated surface protein, transcriptomics, and B cell receptor (BCR) repertoire analysis of sorted plasmablasts and spike⁺ (S-2P⁺) and S-2P^- B cells reveal clonal expansion and accumulating mutations among S-2P⁺ cells. These cells are enriched in a cluster of immunoglobulin G-expressing MBCs and evolve along a bifurcated trajectory rooted in CXCR3⁺ MBCs. One branch leads to CD11c⁺ atypical MBCs while the other develops from CD71⁺ a

SUBMITTER: Lopes de Assis F 

PROVIDER: S-EPMC10529190 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature