Differentiation state and culture conditions impact neural stem/progenitor cell-derived extracellular vesicle bioactivity.

ABSTRACT: Extracellular vesicles (EVs) derived from neural progenitor/stem cells (NPSCs) have shown promising efficacy in a variety of preclinical models. However, NPSCs lack critical neuroregenerative functionality such as myelinating capacity. Further, culture conditions used in NPSC EV production lack standardization, limiting reproducibility challenging and potentially potency of the overall approach via lack of optimization. Here, we assessed whether oligodendrocyte precursor cells (OPCs) and immature oligodendrocytes (iOLs), which are further differentiated than NPSCs and which both give rise to mature myelinating oligodendrocytes, could yield EVs with neurotherapeutic properties comparable or superior to those from NPSCs. We additionally examined the effects of extracellular matrix (EC

SUBMITTER: Dutta D 

PROVIDER: S-EPMC10529403 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature