Discovery of ACE Inhibitory Peptides Derived from Green Coffee Using In Silico and In Vitro Methods.

ABSTRACT: Inhibition of angiotensin-I converting enzyme (ACE) is an important means of treating hypertension since it plays an important regulatory function in the renin-angiotensin system. The aim of this study was to investigate the ACE inhibitory effect of bioactive peptides from green coffee beans using in silico and in vitro methods. Alcalase and thermolysin were employed to hydrolyze protein extract from coffee beans. Bioactive peptides were identified by LC-MS/MS analysis coupled with database searching. The potential bioactivities of peptides were predicted by in silico screening, among which five novel peptides may have ACE inhibitory activity. In vitro assay was carried out to determine the ACE inhibitory degree. Two peptides (IIPNEVY, ITPPVMLPP) were obtained with IC₅₀ values o

SUBMITTER: Dai H 

PROVIDER: S-EPMC10529643 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature