TRPV1 Channels Are New Players in the Reticulum-Mitochondria Ca²⁺ Coupling in a Rat Cardiomyoblast Cell Line.

ABSTRACT: The Ca²⁺ release in microdomains formed by intercompartmental contacts, such as mitochondria-associated endoplasmic reticulum membranes (MAMs), encodes a signal that contributes to Ca²⁺ homeostasis and cell fate control. However, the composition and function of MAMs remain to be fully defined. Here, we focused on the transient receptor potential vanilloid 1 (TRPV1), a Ca²⁺-permeable ion channel and a polymodal nociceptor. We found TRPV1 channels in the reticular membrane, including some at MAMs, in a rat cardiomyoblast cell line (SV40-transformed H9c2) by Western blotting, immunostaining, cell fractionation, and proximity ligation assay. We used chemical and genetic probes to perform Ca²⁺ imaging in four cellular compartments: the endoplasmic ret

SUBMITTER: Tessier N 

PROVIDER: S-EPMC10529771 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature