Towards universal synthetic heterotrophy using a metabolic coordinator.

ABSTRACT: Engineering the utilization of non-native substrates, or synthetic heterotrophy, in proven industrial microbes such as Saccharomyces cerevisiae represents an opportunity to valorize plentiful and renewable sources of carbon and energy as inputs to bioprocesses. We previously demonstrated that activation of the galactose (GAL) regulon, a regulatory structure used by this yeast to coordinate substrate utilization with biomass formation during growth on galactose, during growth on the non-native substrate xylose results in a vastly altered gene expression profile and faster growth compared with constitutive overexpression of the same heterologous catabolic pathway. However, this effort involved the creation of a xylose-inducible variant of Gal3p (Gal3p^Syn4.1), the sensor protein of

SUBMITTER: Sullivan SF 

PROVIDER: S-EPMC10529783 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature