Conditional fluorescent mouse translocation reporters for ERK1/2 and AKT signaling.

ABSTRACT: Understanding how cells activate intracellular signaling pathways in response to external signals, such as growth factors, is a longstanding goal of cell and developmental biology. Recently, live-cell signaling reporters have greatly expanded our understanding of signaling dynamics in response to wide-ranging stimuli and chemical or genetic perturbation, both ex vivo (cell lines) and in vivo (whole embryos or animals). Among the many varieties of reporter systems, translocation reporters that change sub-cellular localization in response to pathway activation have received considerable attention for their ease of use compared to FRET systems and favorable response times compared to transcriptional reporters. We reasoned that mouse reporter lines expressed in a conditional fashion would be a

SUBMITTER: Dinsmore CJ 

PROVIDER: S-EPMC10529872 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature