Conformation-specific Synthetic Antibodies Discriminate Multiple Functional States of the Ion Channel CorA.

ABSTRACT: CorA, the primary magnesium ion channel in prokaryotes and archaea, is a prototypical homopentameric ion channel that undergoes ion-dependent conformational transitions. CorA adopts five-fold symmetric non-conductive states in the presence of high concentrations of Mg²⁺, and highly asymmetric flexible states in its complete absence. However, the latter were of insufficient resolution to be thoroughly characterized. In order to gain additional insights into the relationship between asymmetry and channel activation, we exploited phage display selection strategies to generate conformation-specific synthetic antibodies (sABs) against CorA in the absence of Mg²⁺. Two sABs from these selections, C12 and C18, showed different degrees of Mg²⁺-sensitivity. Through s

SUBMITTER: Erramilli SK 

PROVIDER: S-EPMC10529903 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature