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Quantitative Risk Assessment of Five Foodborne Viruses in Shellfish Based on Multiplex qPCR.


ABSTRACT: Foodborne diseases are currently the most critical food safety issue in the world. There are not many hazard identification and exposure assessments for foodborne viruses (Norovirus GI, GII, Hepatitis A Virus, Rotavirus, Adenovirus) in shellfish. Multiplex qPCR for the simultaneous detection of five foodborne viruses was established and used to assess infection risk based on a 1-year pathogenesis study. The sensitivity, specificity and reproducibility of the multiplex qPCR method are consistent with that of conventional qPCR, which saves more time and effort. Overall, 37.86% of shellfish samples had one or more foodborne viruses. Risk assessment formulae and matrices were used to develop risk assessments for different age groups, different seasons and different shellfish. The annual probab

SUBMITTER: Yu Z 

PROVIDER: S-EPMC10530164 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature