Stacking thick perfusable human microvascular grafts enables dense vascularity and rapid integration into infarcted rat hearts.

ABSTRACT: Fabrication of large-scale engineered tissues requires extensive vascularization to support tissue survival and function. Here, we report a modular fabrication approach, by stacking of patterned collagen membranes, to generate thick (2 mm and beyond), large, three-dimensional, perfusable networks of endothelialized vasculature. In vitro, these perfusable vascular networks exhibit remodeling and evenly distributed perfusion among layers, while maintaining their patterned, open-lumen architecture. Compared to non-perfusable, self-assembled vasculature, constructs with perfusable vasculature demonstrated increased gene expression indicative of vascular development and angiogenesis. Upon implantation onto infarcted rat hearts, perfusable vascular networks attain greater host vascular integrati

SUBMITTER: Zeinstra N 

PROVIDER: S-EPMC10530304 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature