Natural killer cell-mediated contact dermatitis-like reaction induced by treatment with TLR3 ligand poly(I:C).

ABSTRACT:

Introduction

Poly(I:C) is recognised by endosomal Toll-like receptor 3 (TLR3) and activates cytotoxic CD8(+) lymphocytes and natural killer (NK) cells. It has been shown that the viral TLR3 agonist induces robust and long-lasting T-cell-mediated responses. In addition, TLR3 modulates the contact hypersensitivity reaction.

Objective

This study aimed to determine whether poly(I:C) injection can induce NK-mediated hapten reactivity in mice.

Methods

Mice were treated with poly(I:C), and their response to dinitrofluorobenzene hapten was measured by assessing ear swelling and serum interferon gamma (IFN-γ) production. Adoptive cell transfer and cell sorting were used to investigate the mechanism of the reaction, and the phenotype of poly(I:C)-activated liver NK cells was de

SUBMITTER: Majewska-Szczepanik M 

PROVIDER: S-EPMC10530359 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature