Systematic analysis of the impact of phosphorylation and O-GlcNAcylation on protein subcellular localization.

ABSTRACT: The subcellular localization of proteins is critical for their functions in eukaryotic cells and is tightly correlated with protein modifications. Here, we comprehensively investigate the nuclear-cytoplasmic distributions of the phosphorylated, O-GlcNAcylated, and non-modified forms of proteins to dissect the correlation between protein distribution and modifications. Phosphorylated and O-GlcNAcylated proteins have overall higher nuclear distributions than non-modified ones. Different distributions among the phosphorylated, O-GlcNAcylated, and non-modified forms of proteins are associated with protein size, structure, and function, as well as local environment and adjacent residues around modification sites. Moreover, we perform site-mutagenesis experiments using phosphomimetic and phospho

SUBMITTER: Xu S 

PROVIDER: S-EPMC10530397 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature