Human IgM^hiCD300a⁺ B Cells Are Circulating Marginal Zone Memory B Cells That Respond to Pneumococcal Polysaccharides and Their Frequency Is Decreased in People Living with HIV.

ABSTRACT: CD300a is differentially expressed among B cell subsets, although its expression in immunoglobulin (Ig)M⁺ B cells is not well known. We identified a B cell subset expressing CD300a and high levels of IgM (IgM^hiCD300a⁺). The results showed that IgM^hiCD300a⁺ B cells were CD10^-CD27⁺CD25⁺IgD^loCD21^hiCD23^-CD38^loCD1c^hi, suggesting that they are circulating marginal zone (MZ) IgM memory B cells. Regarding the immunoglobulin repertoire, IgM^hiCD300a⁺ B cells exhibited a higher mutation rate and usage of the IgH-VDJ genes than the IgM⁺CD300a^- counterpart. Moreover, the shorter complementarity-determining region 3 (CD

SUBMITTER: Vitalle J 

PROVIDER: S-EPMC10530418 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature