Wnt/β-catenin signaling controls mouse eyelid growth by mediating epithelial-mesenchymal interactions.

ABSTRACT:

Purpose

To investigate the role of Wnt/β-catenin signaling in mouse eyelid development.

Methods

Wnt/β-catenin signaling was disrupted by deleting supraorbital mesenchymal β-catenin or epithelial Wls. p63 was removed to determine whether the expression of Wnts is affected. The eyelid morphology was examined at different stages. Proliferation, apoptosis, and expression of Wnt ligands and their target genes were analyzed via immunofluorescence staining, TUNEL assay, and in situ hybridization.

Results

Deletion of β-catenin in supraorbital mesenchyme abolishes eyelid growth by causing decreased proliferation in supraorbital epithelium and underlying mesenchyme. Inhibition of Wnt secretion by deleting Wls in supraorbital epithelium results in failure of eyelid development,

SUBMITTER: Zhu X 

PROVIDER: S-EPMC10530504 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature