Optical Detection of Distal Lung Enzyme Activity in Human Inflammatory Lung Disease.

ABSTRACT: Objective and Impact Statement. There is a need to develop platforms delineating inflammatory biology of the distal human lung. We describe a platform technology approach to detect in situ enzyme activity and observe drug inhibition in the distal human lung using a combination of matrix metalloproteinase (MMP) optical reporters, fibered confocal fluorescence microscopy (FCFM), and a bespoke delivery device. Introduction. The development of new therapeutic agents is hindered by the lack of in vivo in situ experimental methodologies that can rapidly evaluate the biological activity or drug-target engagement in patients. Methods. We optimised a novel highly quenched optical molecular reporter of enzyme activity (FIB One) and developed a translational pathway

SUBMITTER: Megia-Fernandez A 

PROVIDER: S-EPMC10530652 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature