Synergistic Interaction of 5-HT_1B and 5-HT_2B Receptors in Cytoplasmic Ca²⁺ Regulation in Human Umbilical Vein Endothelial Cells: Possible Involvement in Pathologies.

ABSTRACT: The aim of this work was to explore the involvement of 5-HT_1B and 5-HT_2B receptors (5-HT_1BR and 5-HT_2BR) in the regulation of free cytoplasmic calcium concentration ([Ca²⁺]_i) in human umbilical vein endothelial cells (HUVEC). We have shown by quantitative PCR analysis, that 5-HT_1BR and 5-HT_2BR mRNAs levels are almost equal in HUVEC. Immunofluorescent staining demonstrated, that 5-HT_1BR and 5-HT_2BR are expressed both in plasma membrane and inside the cells. Intracellular 5-HT_1BR are localized mainly in the nuclear region, whereas 5-HT_2BR receptors are almost evenly distributed in HUVEC. 5-HT, 5-HT_1BR agonist CGS12066B, or 5-HT_2BR agonist B

SUBMITTER: Rybakova EY 

PROVIDER: S-EPMC10530667 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature