Unraveling the Kinetics of the 10-23 RNA-Cleaving DNAzyme.

ABSTRACT: DNA-based enzymes, or DNAzymes, are single-stranded DNA sequences with the ability to catalyze various chemical reactions, including the cleavage of the bond between two RNA nucleotides. Lately, an increasing interest has been observed in these RNA-cleaving DNAzymes in the biosensing and therapeutic fields for signal generation and the modulation of gene expression, respectively. Additionally, multiple efforts have been made to study the effects of the reaction environment and the sequence of the catalytic core on the conversion of the substrate into product. However, most of these studies have only reported alterations of the general reaction course, but only a few have focused on how each individual reaction step is affected. In this work, we present for the first time a mathematical mod

SUBMITTER: Montserrat Pages A 

PROVIDER: S-EPMC10531344 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature