Identification of P450 Candidates Associated with the Biosynthesis of Physalin-Class Compounds in Physalis angulata.

ABSTRACT: The Physalis genus has long been used as traditional medicine in the treatment of various diseases. Physalins, the characteristic class of compounds in this genus, are major bioactive constituents. To date, the biogenesis of physalins remains largely unknown, except for the recently established knowledge that 24-methyldesmosterol is a precursor of physalin. To identify the genes encoding P450s that are putatively involved in converting 24-methyldesmosterol to physalins, a total of 306 P450-encoding unigenes were retrieved from our recently constructed P. angulata transcriptome. Extensive phylogenetic analysis proposed 21 P450s that might participate in physalin biosynthesis. To validate the candidates, we developed a virus-induced gene silencing (VIGS) system for P. angula

SUBMITTER: Hua C 

PROVIDER: S-EPMC10531436 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature