METTL3 Promotes the Differentiation of Goat Skeletal Muscle Satellite Cells by Regulating MEF2C mRNA Stability in a m⁶A-Dependent Manner.

ABSTRACT: The development of mammalian skeletal muscle is a highly complex process involving multiple molecular interactions. As a prevalent RNA modification, N6-methyladenosine (m⁶A) regulates the expression of target genes to affect mammalian development. Nevertheless, it remains unclear how m⁶A participates in the development of goat muscle. In this study, methyltransferase 3 (METTL3) was significantly enriched in goat longissimus dorsi (LD) tissue. In addition, the global m⁶A modification level and differentiation of skeletal muscle satellite cells (MuSCs) were regulated by METTL3. By performing mRNA-seq analysis, 8050 candidate genes exhibited significant changes in expression level after the knockdown of METTL3 in MuSCs. Additionally, methylated RNA immunopreci

SUBMITTER: Zhao S 

PROVIDER: S-EPMC10531580 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature