Detection of point mutations associated with resistance of Helicobacter pylori to clarithromycin by hybridization in liquid phase.

ABSTRACT: When the standard procedure for determining antibiotic susceptibility of bacteria is used, the results are delayed, especially for bacteria that grow slowly, such as Helicobacter pylori. Treatment for this bacterium may involve clarithromycin, a compound for which resistance has been associated with point mutations on the 23S rRNA gene. This resistance is currently found in organisms isolated from 0 to 15% of patients and jeopardizes the success of the treatment. We have designed a test involving amplification and colorimetric hybridization in the liquid phase to detect the mutation at the molecular level. First, four reference strains, including the wild type and three strains with the mutations A2143C, A2143G, and A2144G, were used to optimize the method. Amplification was carried out wi

SUBMITTER: Pina M 

PROVIDER: S-EPMC105316 | biostudies-literature | 1998 Nov

REPOSITORIES: biostudies-literature