PLK1 Regulates MicroRNA Biogenesis through Drosha Phosphorylation.

ABSTRACT: Polo-Like Kinase 1 (PLK1), a key mediator of cell-cycle progression, is associated with poor prognosis and is a therapeutic target in a number of malignancies. Putative phosphorylation sites for PLK1 have been identified on Drosha, the main catalytic component of the microprocessor responsible for miR biogenesis. Several kinases, including GSK3β, p70 S6 kinase, ABL, PAK5, p38 MAPK, CSNK1A1 and ANKRD52-PPP6C, have been shown to phosphorylate components of the miR biogenesis machinery, altering their activity and/or localisation, and therefore the biogenesis of distinct miR subsets. We hypothesised that PLK1 regulates miR biogenesis through Drosha phosphorylation. In vitro kinase assays confirmed PLK1 phosphorylation of Drosha at S³⁰⁰ and/or S³⁰². PLK1 inhibition reduce

SUBMITTER: Fletcher CE 

PROVIDER: S-EPMC10531876 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature