Analysis of the stepwise acquisition of bla_CTX-M-2 and subsequent acquisition of either bla_IMP-1 or bla_IMP-6 in highly conserved IncN-pST5 plasmids.

ABSTRACT:

Objectives

ESBL and carbapenemase genes in Enterobacterales spread via plasmids. Nosocomial outbreaks caused by Enterobacterales producing both CTX-M-2 and either IMP-1 or IMP-6-type carbapenemases have been reported. These organisms carry the incompatibility type N plasmid belonging to plasmid ST 5 (IncN-pST5). We investigated the construction process of the ESBL and carbapenemase genes co-carrying IncN-pST5.

Methods

We retrospectively performed draft WGS analysis for bla_IMP- or bla_CTX-M-positive Enterobacterales in our strain collection (n = 281).

Results

We selected four types of Escherichia coli plasmids for our study: type A, which carries both bla_CTX-M-2 and bla_IMP-1 (n 

SUBMITTER: Ikegaya K 

PROVIDER: S-EPMC10532110 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature