High Recovery Chromatographic Purification of mRNA at Room Temperature and Neutral pH.

ABSTRACT: Messenger RNA (mRNA) is becoming an increasingly important therapeutic modality due to its potential for fast development and platform production. New emerging RNA modalities, such as circular RNA, drive the need for the development of non-affinity purification approaches. Recently, the highly efficient chromatographic purification of mRNA was demonstrated with multimodal monolithic chromatography media (CIM^® PrimaS), where efficient mRNA elution was achieved with an ascending pH gradient approach at pH 10.5. Here, we report that a newly developed chromatographic material enables the elution of mRNA at neutral pH and room temperature. This material demonstrates weak anion-exchanging properties and an isoelectric point of 5.3. It enables the baseline separation of mRNA (at least

SUBMITTER: Miklavcic R 

PROVIDER: S-EPMC10532270 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature