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Evolution of CRISPR/Cas Systems for Precise Genome Editing.

ABSTRACT: The bacteria-derived CRISPR/Cas (an acronym for regularly interspaced short palindromic repeats/CRISPR-associated protein) system is currently the most widely used, versatile, and convenient tool for genome engineering. CRISPR/Cas-based technologies have been applied to disease modeling, gene therapies, transcriptional modulation, and diagnostics. Nevertheless, some challenges remain, such as the risk of immunological reactions or off-target effects. To overcome these problems, many new methods and CRISPR/Cas-based tools have been developed. In this review, we describe the current classification of CRISPR systems and new precise genome-editing technologies, summarize the latest applications of this technique in several fields of research, and, finally, discuss CRISPR/Cas system limitations, ethical issues, and challenges.

SUBMITTER: Hryhorowicz M

PROVIDER: S-EPMC10532350 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature

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Evolution of CRISPR/Cas Systems for Precise Genome Editing.

Hryhorowicz Magdalena M Lipiński Daniel D Zeyland Joanna J

International journal of molecular sciences 20230918 18

The bacteria-derived CRISPR/Cas (an acronym for regularly interspaced short palindromic repeats/CRISPR-associated protein) system is currently the most widely used, versatile, and convenient tool for genome engineering. CRISPR/Cas-based technologies have been applied to disease modeling, gene therapies, transcriptional modulation, and diagnostics. Nevertheless, some challenges remain, such as the risk of immunological reactions or off-target effects. To overcome these problems, many new methods ...[more]

PMID: 37762535

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