Calmodulin, Calcium/Calmodulin-Dependent Kinases-1 and 2 Regulate Expression of the Heat Shock Proteins for Heat Shock Tolerance and Pheromone Signaling Genes for Sexual Development in Neurospora crassa.

ABSTRACT: Calmodulin (CaM) is a primary Ca²⁺ sensor that binds and activates numerous target proteins and regulates several cellular processes in eukaryotes. CaM is essential in Neurospora crassa; therefore, we generated a CaM mutant using repeat-induced point (RIP) mutation and investigated the cmd^RIP mutant phenotypes. We also studied knockout mutants of four Ca²⁺/CaM kinases (camk-1, 2, 3, and 4) for their role during stress conditions and sexual development. The cmd^RIP, ∆camk-1, and ∆camk-2 mutants showed reduced survival and growth rates under heat stress, oxidative stress, pH, and ER stress conditions. In addition, under the heat stress conditions, expression of the heat shock protein gen

SUBMITTER: Marak CNK 

PROVIDER: S-EPMC10533439 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature