Lung extracellular matrix modulates KRT5⁺ basal cell activity in pulmonary fibrosis.

ABSTRACT: Aberrant expansion of KRT5⁺ basal cells in the distal lung accompanies progressive alveolar epithelial cell loss and tissue remodelling during fibrogenesis in idiopathic pulmonary fibrosis (IPF). The mechanisms determining activity of KRT5⁺ cells in IPF have not been delineated. Here, we reveal a potential mechanism by which KRT5⁺ cells migrate within the fibrotic lung, navigating regional differences in collagen topography. In vitro, KRT5⁺ cell migratory characteristics and expression of remodelling genes are modulated by extracellular matrix (ECM) composition and organisation. Mass spectrometry- based proteomics revealed compositional differences in ECM components secreted by primary human lung fibroblasts (HLF) from IPF patients compared to co

SUBMITTER: Hewitt RJ 

PROVIDER: S-EPMC10533905 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature