Live imaging of microglia during sleeping sickness reveals early and heterogeneous inflammatory responses.

ABSTRACT:

Introduction

Invasion of the central nervous system (CNS) is the most serious consequence of Trypanosoma brucei infection, which causes sleeping sickness. Recent experimental data have revealed some more insights into the disease during the meningoencephalitic stage. However, detailed cellular processes befalling the CNS during the disease are poorly understood.

Methods

To further address this issue, we implanted a cranial window on the cortex of B6.129P2(Cg)-Cx3cr1^tm1Litt/J mice, infected them with Trypanosoma brucei expressing RFP via intraperitoneal injection, and monitored microglial cells and parasites longitudinally over 30 days using in vivo 2-photon imaging. We correlated the observed changes with histological analyses to evalua

SUBMITTER: Uzcategui NL 

PROVIDER: S-EPMC10534015 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature