Determination of 73 multi-class pesticides in okra (Abelmoschus esculentus L.) fruits using LC-MS/MS and GC-MS/MS and estimation of analytical uncertainty of measurement.

ABSTRACT: This study developed a method to simultaneously determine 73 multi-class pesticides in okra fruit using LC-MS/MS and GC-MS/MS. The sample was extracted with acetonitrile and subsequent clean-up through dispersive-SPE method. The quantification level of the technique was 0.01 µg g^-1 and compliance to the MRLs fixed by the regulatory bodies like EU and FSSAI. The recovery at 10, 50, and 100 µg kg^-1 spiked levels; intra and inter-day precision at 50 µg kg^-1 were found within 70-120% with RSD less than 15% with LC-MS/MS and GC-MS/MS. Measurement uncertainty was in the range of 1.81 to 12.91 µg kg^-1 estimated at 50 µg kg^-1. The matrix effects were slightly higher for LC than GC-compatible pesticides. Risk assessment for pesticides detected

SUBMITTER: Pallavi MS 

PROVIDER: S-EPMC10534163 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature