A Comparative Analysis of the Stomach, Gut, and Lung Microbiomes in Rattus norvegicus.

ABSTRACT: Urban rats serve as reservoirs for several zoonotic pathogens that seriously endanger public health, destroy stored food, and damage infrastructure due to their close interaction with humans and domestic animals. Here, we characterize the core microbiomes of R. norvegicus's stomach, gut, and lung using 16S rRNA next-generation Illumina HiSeq sequencing. The USEARCH software (v11) assigned the dataset to operational taxonomic units (OTUs). The alpha diversity index was calculated using QIIME1, while the beta diversity index was determined using the Bray-Curtis and Euclidean distances between groups. Principal component analyses visualized variation across samples based on the OTU information using the R package. Linear discriminant analysis, effect sizes (LEfSe), and phylogenetic inv

SUBMITTER: Shah T 

PROVIDER: S-EPMC10534326 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature