Optimization of Rhodococcus erythropolis JCM3201^T Nutrient Media to Improve Biomass, Lipid, and Carotenoid Yield Using Response Surface Methodology.

ABSTRACT: The oleaginous bacterium Rhodococcus erythropolis JCM3201^T offers various unique enzyme capabilities, and it is a potential producer of industrially relevant compounds, such as triacylglycerol and carotenoids. To develop this strain into an efficient production platform, the characterization of the strain's nutritional requirement is necessary. In this work, we investigate its substrate adaptability. Therefore, the strain was cultivated using nine nitrogen and eight carbon sources at a carbon (16 g L^-1) and nitrogen (0.16 g L^-1) weight ratio of 100:1. The highest biomass accumulation (3.1 ± 0.14 g L^-1) was achieved using glucose and ammonium acetate. The highest lipid yield (156.7 ± 23.0 mg g^-1_DCW) was achieved using

SUBMITTER: Engelhart-Straub S 

PROVIDER: S-EPMC10534354 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature