L-Asparaginase Type II from Fusarium proliferatum: Heterologous Expression and In Silico Analysis.

ABSTRACT: The search for new drug-producing microorganisms is one of the most promising situations in current world scientific scenarios. The use of molecular biology as well as the cloning of protein and compound genes is already well established as the gold standard method of increasing productivity. Aiming at this increase in productivity, this work aims at the cloning, purification and in silico analysis of l-asparaginase from Fusarium proliferatum in Komagataella phaffii (Pichia pastoris) protein expression systems. The l-asparaginase gene (NCBI OQ439985) has been cloned into Pichia pastoris strains. Enzyme production was analyzed via the quantification of aspartic B-hydroxamate, followed by purification on a DEAE FF ion exchange column. The in silico analysis was proposed

SUBMITTER: Cardoso SL 

PROVIDER: S-EPMC10534586 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature