The Identification of Viral Pathogens in a <i>Physostegia virginiana</i> Plant Using High-Throughput RNA Sequencing.

ABSTRACT: Physostegia virginiana is an important ornamental and cut-flower plant in China. Its commonly used method of clonal propagation leads to virus accumulation in this plant. However, which viruses can infect the Physostegia virginiana plant remains to be illuminated. In this work, five viral pathogens in a Physostegia virginiana plant with virus-like symptoms of yellow, shriveled, and curled leaves were identified using RNA-seq, bioinformatics, and molecular biological techniques. These techniques allowed us to identify five viruses comprising one known alfalfa mosaic virus (AMV) and four novel viruses. The novel viruses include a virus belonging to the genus Fabavirus, temporarily named Physostegia virginiana crinkle-associated virus 1 (PVCaV1); two viruses

SUBMITTER: Dong J 

PROVIDER: S-EPMC10534606 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature