Surface Acoustic Wave Immunosensor for Detection of Botulinum Neurotoxin.

ABSTRACT: A Love-type acoustic wave sensor (AT-cut quartz substrate, SiO₂ guiding layer) with a center frequency of approximately 120 MHz was used to detect a simulant of pathogenic botulinum neurotoxin type A-recombinant of BoNT-A light chain-in liquid samples. The sensor was prepared by immobilizing monoclonal antibodies specific for botulinum neurotoxin via a thiol monolayer deposited on a gold substrate. Studies have shown that the sensor enables selective analyte detection within a few minutes. In addition, the sensor can be used several times (regeneration of the sensor is possible using a low pH buffer). Nevertheless, the detectability of the analyte is relatively low compared to other analytical techniques that can be used for rapid detection of botulinum neurotoxin. The obtained

SUBMITTER: Grabka M 

PROVIDER: S-EPMC10534944 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature