Investigation of Peptides for Molecular Recognition of C-Reactive Protein-Theoretical and Experimental Studies.

ABSTRACT: We investigate the interactions between C-reactive protein (CRP) and new CRP-binding peptide materials using experimental (biological and physicochemical) methods with the support of theoretical simulations (computational modeling analysis). Three specific CRP-binding peptides (P2, P3, and P9) derived from an M13 bacteriophage have been identified using phage-display technology. The binding efficiency of the peptides exposed on phages toward the CRP protein was demonstrated via biological methods. Fibers of the selected phages/peptides interact differently due to different compositions of amino acid sequences on the exposed peptides, which was confirmed by transmission electron microscopy. Numerical and experimental studies consistently showed that the P3 peptide is the best CRP binder. A

SUBMITTER: Szot-Karpinska K 

PROVIDER: S-EPMC10535004 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature