Single-cell analysis of 5-aminolevulinic acid intraoperative labeling specificity for glioblastoma.

ABSTRACT:

Objective

Glioblastoma (GBM) is the most common and aggressive malignant primary brain tumor, and resection is a key part of the standard of care. In fluorescence-guided surgery (FGS), fluorophores differentiate tumor tissue from surrounding normal brain. The heme synthesis pathway converts 5-aminolevulinic acid (5-ALA), a fluorogenic substrate used for FGS, to fluorescent protoporphyrin IX (PpIX). The resulting fluorescence is believed to be specific to neoplastic glioma cells, but this specificity has not been examined at a single-cell level. The objective of this study was to determine the specificity with which 5-ALA labels the diversity of cell types in GBM.

Methods

The authors performed single-cell optical phenotyping and expression sequencing-version 2 (SCOPE-seq2), a

SUBMITTER: Liu Z 

PROVIDER: S-EPMC10535619 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature