Determination of Polycyclic Aromatic Hydrocarbons (PAHs) and Phthalates in Human Placenta by Mixed Hexane/Ether Extraction and Gas Chromatography-Mass Spectrometry/Mass Spectrometry (GC-MS/MS).

ABSTRACT: In this study, we evaluated the extraction effect of three different extractants, namely hexane + ether (v/v = 3:1), acetonitrile and ethyl acetate, on polycyclic aromatic hydrocarbons (PAHs) and phthalic acid esters (PAEs) in placenta detected and analysed by triple quadrupole gas chromatography-mass spectrometry (GC-MS/MS). The results showed that n-hexane + ether (v/v = 3:1) had the highest extraction efficiency. Under the optimal conditions, the limits of detection (LOD) for the 10 PAHs were 0.003-0.0167 μg/L with relative standard deviations (RSD) of 1.4-5.48% and detection rates of 68.19-107.05%, and the correlation coefficients were (R², 0.9982-0.9999). The LODs for the nine PAEs were 0.0015-3.5714 μg/L and the correlation coefficients were (R<

SUBMITTER: Tao L 

PROVIDER: S-EPMC10536546 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature