Boron Clusters Alter the Membrane Permeability of Dicationic Fluorescent DNA-Staining Dyes.

ABSTRACT: Membrane-permeable fluorescent dyes that stain DNA are useful reagents for microscopic imaging, as they can be introduced into living cells to label DNA. However, the number of these dyes, such as Hoechst 33342, is limited. Here, we show that the icosahedral dodecaborate B₁₂Br₁₂^2-, a superchaotropic carrier that delivers different types of molecules into cells, functions as an excellent carrier for membrane-impermeable fluorescent dyes. Propidium iodide (PI) and 4',6-diamidino-2-phenylindole (DAPI), dicationic membrane-impermeable fluorescent dyes that stain DNA, can permeate cell membranes in the presence of boron clusters. Methyl green (MG), a dicationic dye used in the histological and fluorescent staining of DNA, permeated cell membranes in the presence

SUBMITTER: Hirai Y 

PROVIDER: S-EPMC10536875 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature