Optimizing the Conditions for Whole-Genome Sequencing of Avian Reoviruses.

ABSTRACT: Whole-genome sequencing (WGS) is becoming an essential tool to characterize the genomes of avian reovirus (ARV), a viral disease of economic significance to poultry producers. The current strategies and procedures used to obtain the complete genome sequences of ARV isolates are not cost-effective because most of the genetic material data resulting from next-generation sequencing belong to the host and cannot be used to assemble the viral genome. The purpose of this study was to develop a workflow to enrich the ARV genomic content in a sample before subjecting it to next-generation sequencing (NGS). Herein, we compare four different ARV purification and enrichment approaches at the virion, RNA and cDNA levels to determine which treatment or treatment combination would provide a higher propo

SUBMITTER: Narvaez SA 

PROVIDER: S-EPMC10536876 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature