Highly sensitive and rapid determination of Mycobacterium leprae based on real-time multiple cross displacement amplification.

ABSTRACT:

Background

Mycobacterium leprae (ML) is the pathogen that causes leprosy, which has a long history and still exists today. ML is an intracellular mycobacterium that dominantly induces leprosy by causing permanent damage to the skin, nerves, limbs and eyes as well as deformities and disabilities. Moreover, ML grows slowly and is nonculturable in vitro. Given the prevalence of leprosy, a highly sensitive and rapid method for the early diagnosis of leprosy is urgently needed.

Results

In this study, we devised a novel tool for the diagnosis of leprosy by combining restriction endonuclease, real-time fluorescence analysis and multiple cross displacement amplification (E-RT-MCDA). To establish the system, primers for the target gene RLEP were designed, and the optimal conditions f

SUBMITTER: Huang J 

PROVIDER: S-EPMC10537127 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature