Synthesis of isobutanol using acetate as sole carbon source in Escherichia coli.

ABSTRACT:

Background

With concerns about depletion of fossil fuel and environmental pollution, synthesis of biofuels such as isobutanol from low-cost substrate by microbial cell factories has attracted more and more attention. As one of the most promising carbon sources instead of food resources, acetate can be utilized by versatile microbes and converted into numerous valuable chemicals.

Results

An isobutanol synthetic pathway using acetate as sole carbon source was constructed in E. coli. Pyruvate was designed to be generated via acetyl-CoA by pyruvate-ferredoxin oxidoreductase YdbK or anaplerotic pathway. Overexpression of transhydrogenase and NAD kinase increased the isobutanol titer of recombinant E. coli from 121.21 mg/L to 131.5 mg/L under batch cultivation. Further optimizatio

SUBMITTER: Gu P 

PROVIDER: S-EPMC10537434 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature