Artificial switches induce the bespoke production of functional compounds in marine microalgae Chlorella by neutralizing CO₂.

ABSTRACT: To improve the CO₂ tolerance of a marine microalga Chlorella sp. of which the production capacity has been demonstrated industrially, a mutant library was created and a strain hct53 was screened. Compared to the parental strain, hct53 shows a high CO₂ capture capacity, while starch biosynthesis is compromised, with increases in health beneficial metabolites and antioxidant capacity. Global gene expression and genome-wide mutation distribution revealed that transcript choreography was concomitant with more active CO₂ sequestration, an increase in the lipid synthesis, and a decrease in the starch and protein synthesis. These results suggest that artificial trait improvement via mutagenesis, couple with multiomics analysis, helps discover genetic switches that induce the bespoke conversion of carbon flow from "redundant metabolites" to valuable ones for functional food.

SUBMITTER: Gu J 

PROVIDER: S-EPMC10537470 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature